Spermatozoa Abnormality Test of Boer Goats Frozen Semen with the Addition of Sweet Orange Essential Oil and Streptomycin in Tris Yolk Extender
Sukma Aditya Sitepu *
Department of Animal Husbandry, Faculty of Science and Technology, Pembangunan Panca Budi University, Medan, Indonesia.
*Author to whom correspondence should be addressed.
Abstract
This research aims to to determine the percentage value of spermatozoa abnormalities in Boer Goat frozen semen by adding a combination of streptomycin with sweet orange essential oil to tris yolk extender. The ingredients used in this study were fresh semen from Boer Goat, tris yolk extender, streptomycin, and sweet orange essential oil. Tris yolk extender is made using Tris (hydroxymethylaminomethane) (3.32 g), citric acid (1.86 g), fructose (1.37 g), glycerol (6 ml), egg yolk (20 ml), aquabides (100 ml). The study design was a non factorial completely treatments given were the addition of sweet orange essential oil at 0%, 0.25%, 0.5%, 0.75% and 1%. The results showed that the addition of a combination of streptomycin and sweet orange essential oil into the tris yolk extender had a highly significant effect (P <0.01) on the percentage value of spermatozoa abnormalities of Boer Goats before and after freezing. The best average value of spermatozoa abnormalities is the addition of sweet orange essential oil at 1% (P4) with the percentage of spermatozoa abnormalities before freezing at 3% and after freezing at 6%.
Keywords: Boer goat, essential oil, frozen semen, streptomycin, sweet orange, spermatozoa abnormality test.