Differential Response of Cotton Growth, Yield and Fiber Quality to Foliar Application of Spirulina platensis and Urea Fertilizer

Main Article Content

Youssef G. Yanni
Amany A. Elashmouny
Abdelgawad Y. Elsadany


Aims: Evaluation if foliar spray of destructed cells of Spirulina platensis along with urea fertilizer can contribute to cotton (Gossypium barbadense L. cultivar Giza-94) plant growth, contents of photosynthetic pigments, seed cotton yield, seed index, earliness, lint percent, fineness (Micronair) and strength (Pressely).

Study Design: Application of ascending rates of N fertilizer in 3 mainplot treatments and foliar spray with destructed cells of Spirulina platensis in 4 subplot treatments (Contained a non-treated control), with 3 replications.

Place and Duration of Study: The Departments of Microbiology and Cotton Research, Sakha Agricultural Research Station, Kafr Elsheikh, Egypt (31º18′30″N & 30º48′14″E) during the two cotton cultivation seasons of years 2016 and 2017.

Methodology: Urea (46% N) at 96, 144 or 192 kg N/ha was applied in two equal doses as main-plot treatments. Spirulina platensis was grown and total proteins, contents of 16 amino acids and Fe, K, Mn and P of dried destructed cells were determined and used for foliar application in the rates of 6, 12 or 18 g cells/ha, used as sub-plot treatments. Area of each replicated sub-plot was 12.5 m2. Calcium super phosphate (15% P2O5), potassium sulphate (48% K2O) and Insecticides (when necessary) were supplemented as common treatments. 

Results: Application of destructed Spirulina increased the plant leaf area, chlorophyll a, b, carotenoids and gibberellin contents, plant height, fruiting branches, open bolls/plant, seed cotton yield and seed index after ginning, fiber length, fineness (Micronair) and strength (Pressely) comparing to the corresponding non-sprayed plants. Urea at 192 kg N/ha along with destructed Spirulina exhibited superiority as enhancers of plant growth, yield and fiber qualities.

Conclusion: The dual N/Spirulina combination expressed enhanced cotton growth, yield and qualities of fibers. The tested fertilization strategy is promising for enhanced cotton production economy.

Cotton, fiber length, fineness and strength, urea, photosynthetic pigments, Spirulina.

Article Details

How to Cite
G. Yanni, Y., A. Elashmouny, A., & Y. Elsadany, A. (2020). Differential Response of Cotton Growth, Yield and Fiber Quality to Foliar Application of Spirulina platensis and Urea Fertilizer. Asian Journal of Advances in Agricultural Research, 12(1), 29-40. https://doi.org/10.9734/ajaar/2020/v12i130072
Original Research Article


Khan A, Tan DKY, Fazal M, Afredi MZ, Shah F, Wei F, et al. Nitrogen nutrition in cotton and control strategies for greenhouse gas emissions: A review. Environmental Science and Pollution Research. 2017;24:23471–23487.

Tamoi M, Nagaoka M, Miyagawa Y, Shigeoka S. Contribution of fructose-1, 6-bisphosphatase and sedoheptulose-1, 7-bisphosphatase to the photosynthetic rate and carbon flow in the Calvin cycle in transgenic plants. Plant Cell Physiol. 2006;47:380-390.

Ronga D, Biazzi E, Parati K, Carminati D, Carminati E, Tava A.. Microalgal biostimulants and biofertilisers in crop productions. Agronomy. 2019;9:1-22.

Khan SA, Hussain MZ, Prasad S, Banerjee UC. Prospects of biodiesel production from microalgae in India. Renew. Sustain. Energy Rev. 2009;13:2361–2372.

Garcia-Gonzalez J, Sommerfeld M. Biofertilizer and biostimulants properties of the microalga Acutodesmus dimorphus. J Appl Phycol. 2016;28:1051–1061.

Rouphael Y, Colla G. Synergistic biostimulatory action: Designing the next generation of plant biostimulants for sustainable agriculture. Front Plant Sci. 2018;9:1-6.

Howard DD, Gwathmey CO, Essington ME, Roberts RK, Mullen MD. Nitrogen fertilization of no-till cotton on loss-derived soils. Agron J. 2001;93:157-163.

Ordog V. Beneficial effects of microalgae and cyanobacteria in plant/soil systems, with special regards to their auxin- and cytokine- like activity. International Workshop and Training Course on Microalgal Biology and Biotechnology. Hungary. 1999;13-26.

Jackson ML. Soil chemical analysis. Prentic-Hall, Inc, Englewood Cliffs, NJ, USA; 1973.

Zarrouk C. Contribution a L’etude D’une Cyanophycee: Influence de divers facteurs physiques et chimiques sur la croissance et la photosynthese de spirulina maxima (Setch et Gardner) Geitler. Ph.D. Thesis, Faculte des Sciences de l’Universite de Paris, Paris, France; 1966.

Randhir S, Pradhan K. Forage evaluation, first published, Printox, New Delhi, Dhawan Printing Works; 1981.

AOAC. Official Methods of Analysis. Association of Official Analytical Chemists 19th Edition, Washington, DC. USA; 2012.

Pise NM, Sabale AB. Effect of seaweed concentrates on the growth and biochemical constituents of Trigonella foenum- graecum L. J. Phytology. 2010; 2:50-56.

Grzesik M, Zdzisława Romanowska-Duda. Ability of cyanobacteria and green algae to improve metabolic activity and develop-ment of willow plants. Pol. J. Environ. Stud. 2015;24:1003-1012.

Wettstein VD. Chlorophyll letale and der sub-mikroskopishe formweschselder plastiden. Exp Cell Res. 1957;12:427.

Shindy WW, Smith O. Identification of plant hormones from cotton ovules. Plant Physiol. 1975;55:550-554.
(Accessed 18 December 2019)

Buckley DJ, Mc Adam WE. A television-scanned, digital planimeter and dimension gage for measuring size of agricultural produce. Trans. Amer. Soc. Agr. Eng. 1972;15:324-326.

ASTM. American stander for testing materials. 1986; ASTM D: 1440-50, D:1578- 67, D:1425; 1986.

Duncan DB. Multiple range and multiple F-Test. Biometrics 1955;11:1-5.

Snedecor GW, Cochran WG. Statistical methods. 6th Edition, Ames, Iowa, the Iowa State University; 1967.

Aly M H A, Abd El-All Azza AM, Mostafa Soha SM. Enhancement of sugar beet seed germination, plant growth, performance and biochemical compounds as contributed by algal extracellular products. J Agric Sci Mansoura Univ. 2008;33(12):8429-8448.

Yassen AA, Abou EL, Nour EAA, Abou Seeda MA, Abdallah MMS, El- Sayed SAA. Effect of potassium fertilization levels and algae extract on growth, bulb yield and quality of onion (Allium cepa L.). Middle East Journal of Agriculture Research. 2018;7:625-638.

Godlewska Michalak KL, Pacyga P, Baśladyńska S, Chojnacka K. Potential applications of cyanobacteria: Spirulina platensis filtrates and homogenates in agriculture. World J Microbiol Biotechnol. 2019;35:1-18.

Rizwan M, Ali S, Hussain A, Ali Q, Shakoor MB, Zia-ur-Rehman M et al. Effect of zinc-lysine on growth, yield and cadmium uptake in wheat (Triticum aestivum L.) and health risk assessment. Chemosphere. 2017;187:35-42.

Bashir A, Rizwan M, Ali S, Zia-ur Rehman MZ, Ishaque W, et al. Effect of foliar-applied iron complexed with lysine on growth and cadmium (Cd) uptake in rice under Cd stress. Environ Sci Pollut Res Int. 2018;25:20691–20699.

Hussain A., Ali S, Rizwan M, Zia-ur Rehman MZ, Hameed AA, Hafeez F, et al. Role of zinc– lysine on growth and chromium uptake in rice plants under Cr stress. J Plant Growth Regul. 2018;37: 1413-1422.

Amin A, Gharib F, El-Awadi M, Rashad E. Physiological response of onion plants to foliar application of putrescine and glutamine. Sci Hortic. 2011;129:353-360.

Khalilzadeh R, Tajbakhsh M, Jalilian J. Effect of foliar application of bio-organic fertilizers and urea on yield and yield components characteristics of mung bean. International Journal of Agriculture Research and Review. 2012;2:639-645.

Kakkar R, Nagar P, Ahuja P, Rai V. Polyamines and plant morphogenesis. Biologia Plantarum. 2000;43:1-11.

Mohamed E, Mohamed S. Improvement the growth and quality of green onion (Allium cepa L.) plants by some bioregulators in the new reclaimed area at Nobaria region, Egypt. New York Science Journal. 2012;5:114-120.

Souri MK, Hatamian M. Aminochelates in plant nutrition: a Review. J Plant Nutr. 2019;42:67-78.

Fawzy Z, El-Sha Z, Yunsheng L, Zhu O, Sawan O. Response of garlic (Allium Sativum L) plants to foliar spraying of some bio-stimulants under sandy soil condition. J Appl Sci Res. 2012;8:770-776.

Talaat I, Bekheta M, Mahgoub M. Physiological response of periwinkle plants (Catharanthus roseus L.) to tryptophan and putrescine. Int J Agric Biol. 2005;7:210-213.

Chen M, Cheng B, Zhang Q, Ding Y, Yang Z. Effects of applying L-methionine, L-phenylalanine and L-tryptophan on Zea mays growth and its nutrient uptake. J Appl Ecol. 2005;16:1033-1037.

El-Awadi M, El-Bassiony A, Fawzy Z, and El-Nemr M. Response of snap bean (Phaseolus vulgaris Lants) to nitrogen fertilizer and foliar application with methionine and tryptophan. Nature and Science. 2011;9:87-94.

Dahab T, El-Aziz N. Physiological effect of diphenylamine and tryptophan on the growth and chemical constituents of Philodendron erubescens plants. World Journal of Agricultural Sciences. 2006; 2:75-81.

Boopathi T, Balamurugan V, Gopinath S, Sundararaman M. Characterization of IAA production by the mangrove cyanobacterium Phormidium sp. MI405019 and its Influence on Tobacco Seed germination and organogenesis. J Plant Growth Regul. 2013;32:758-766.

Romero I, Téllez J, Yamanaka L, Steindel M, Romanha A, Grisard EC. Transsul-furation is an active pathway for cysteine biosynthesis in Trypanosoma rangeli. Parasit Vectors. 2014;7:2-11.

Noroozlo YA, Souri MK, Delshad M. Stimulation effects of foliar applied glycine and glutamine amino acids on Lettuce Growth. Open Agric.2019;4:164-172.

Mohammadipour N, Souri MK. Effects of different levels of glycine in the nutrient solution on the growth, nutrient composition and antioxidant activity of coriander (Coriandrum sativum L.). Acta Agrobot. 2019a;72:6-9.

Mohammadipour N, Souri M.K. Beneficial effects of glycine on growth and leaf nutrient concentrations of coriander (Coriandrum sativum) plants. J Plant Nutr 2019b;42:1637-1644.

Zhenan H, Pinfang L, Baoguo L, Jiang G, Yanna W. Effects of fertigation scheme on N uptake and N use efficiency in cotton. Plant Soil. 2001;290:115–126.

Rodríguez AA, Stella AM, Storni MM, Zulpa G, Zaccaro MC. Effects of cyanobacterial extracellular products and gibberellic acid on salinity tolerance in Oryza sativa L. Aquat Biosyst. 2006;2:7.
(Accessed 18 December 2019)