Insecticidal Property of Black Seed (Nigella sativa) Powder as an Eco-friendly Management of Skin Beetle Dermestes maculatus (Coleoptera: Dermestidae) in Atlantic Codfish Gadus morhua (Gadiformes: Gadidae)

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Bob-Manuel, R. Bekinwari
Ukoroije, R. Boate


The bio-pesticidal potential of Nigella sativa seed powder in the management of Dermestes maculatus in codfish (Gadus morhua) was evaluated in the laboratory. D. maculatus beetles were obtained from naturally infested smoked fish, cultured at ambient temperature for the establishment of new stock and same age adults. Purchased N. sativa seeds were ground into fine powder, weighed at 0.4 g, 0.8 g, 1.2 g, 1.6 g and 2.0 g for use in the bioassay. The treatments were separately added into 40 g codfish kept in Kilner jar into which two sexed pairs of D. maculatus were introduced and observed. From the results, the number of the developmental stages (larvae, pupae and adults) of D. maculatus in codfish treated with N. sativa seed powder was inversely proportional to the concentration of the seed powder. Thus, an increase in the concentration of N. sativa powder generated reduction in the mean number of D. maculatus progeny found in the codfish after 35 days as follows: at 0.4 g, progeny development was (103.50, 7.75, 2.50) and 77.00, 8.25 and 1.00 at 2.0 g respectively for larva, pupa and adult stages. Percentage protection conferred by the botanical on D. maculatus showed that all the doses applied were effective. Corrected mortality of D. maculatus adults after 45 days of exposure to the different doses of N. sativa treatments also increased with an increase in concentration of N. sativa from 0.4 g to 2.0 g. Frass weight generated by D. maculatus activity in codfish treated with N. sativa powder was high in codfish treated at 1.6 g. Finally, N. sativa characterized both qualitatively and quantitatively for phytochemicals, proved the secondary metabolites with bio-pesticide potentials against D. maculatus such as phenols, Alkaloid, flavonoid, terpenoid, saponin, tannin, Cardiac glycoside, anthraquinone, steroid and oxalate in different proportion, therefore, should be utilized in Integrated Pest Management program of stored codfish.

Nigella sativa, Dermestes maculatus, codfish, progeny emergence, phytochemicals.

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How to Cite
Bekinwari, B.-M. R., & Boate, U. R. (2020). Insecticidal Property of Black Seed (Nigella sativa) Powder as an Eco-friendly Management of Skin Beetle Dermestes maculatus (Coleoptera: Dermestidae) in Atlantic Codfish Gadus morhua (Gadiformes: Gadidae). Asian Journal of Advances in Agricultural Research, 13(3), 47-56.
Original Research Article


Peacock ER. Dermestes peruvianus Cast., D. heamorrhoidalis Kust and other Dermestes species. (Coleoptera: Dermestedae). Entomol. 1975;111:1-14.

Adewuyi SA, Okuneye PA, Adeosun IA. and Alaba OM. Profit efficiency in fish smoking in Ilaje local government area of Ondo State, Nigeria. International Journal of Agriculture and Food Science. 2013;4: 421-427.

El-shaika AF, Ray R, Mintet D, Panda S. and Worawattanamateekul W. African fermented fish products in scope of risks. International Food Research Journal. 2014;21(1):425-432.

Thorarinsdottir KA, Arason S, Bogason SG. and Kristbergsson K. The effects of various salt concentrations during brine curing of cod (Gadus morhua). International Journal of Food Science and Technology. 2004;39:79-88.

Dale P, Uwonkunda V. Nigeria’s love affair with a Norwegain fish. BBC News; 2017.

Frederick F. The outsider: My Life in Intrigue. NY: Putnam. 1969;196.

Tidwell JH and Allan GL. Fish as food: aquaculture’s contribution, ecological and economic impacts and contributions of fish farming and capture fisheries. EMBO Reports. 2001:2(11):958-963.

Berr C, Akbaraly T, Arnaud J, Hininger I, Roussel AM, Barberger GP. Increased selenium intake in elderly high fish consumers may account for health benefits previously ascribed to omega-3 fatty acids. 2009;315-318.

Haines CP, Rees DP. Demestes SPP. A field guide to the types of insects and mites infesting cured fish. FAO, (1981). The prevention of losses in cured fish. Tech, pap. 1989;219: 87.

Zakka U, Dimkpa SON, Lale NES. Morphometric studies of different developmental stages of Dermestes maculatus (DeGeer, 1774) (Coleoptera: Dermestidae). Current Research Journal of Biological Sciences. 2009;1(3): 99-101.

FAO, The prevention of losses in cured fish. Tech, pap. 1981;219:87.

Raja N. Botanicals: Sources for eco-friendly biopesticides. J. Biofertil. Biopestic. 2014;5:e122.

Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global J. Pure Applied Sci. 2002;8:203-208.

Karpagam T, Devaraj A. Studies on the efficacy of Aloe vera on antimicrobial activity. Int. J. Res. Ayurveda and Pharm. 2011;4:1286–1289.

Mejule FO, Akinwande OA, Fajemisin OO. Screening of West African black pepper (Piper guineense) for insecticidal and fungicidal properties. 24th Annual Report of Nigerian Store Prod. Res. Inst. 1987;12: 87-92.

Thiruppathi S, Ramasubramanian V, Sivakumar T, Thirumalai AV. Antimicrobial activity of Aloe vera (L.) Burm. F. against pathogenic microorganisms. J. Biosci. Res. 2010;4:251–258.

Akinwumi FA. Adedire CO, Fasakin EA. Effect of four plant materials on Egg hatchability of Dermestes maculatus and Necrobia rufipes on smoked African mud catfish Clarias gariepinus, Burchell. Science Research Annals. 2006b;21:38-42.

Sarwar M. Microbial insecticides- an eco-friendly effective line of attack for insect pest management. Int. J. Eng. Adv. Res. Technol. 2015;2:4–9.

Castillo-Sánchez LE, Jiménez-Osornio JJ, Delgado-Herrera MA, Candelaria-Martínez, B, Sandoval-Gío JJ. Effects of the hexanic extract of neem Azadirachta indica against adult whitefly Bemisia tabaci. J. Entomol. Zool. Stud. 2015;5:95–99.

Jansen PCM. Spices, condiments and medicinal plants in Ethiopia, their taxonomy and agricultural significance. Agric. Res. Rep. 1981;906.

ISBN 90-220-0767-7.

Joseph B, Sujatha S. Insight of botanical based biopesticides against economically important pest. Int. J. Pharm. Life Sci. 2012;11:2138–2148.

Sharafzadeh S. Pyrethrum, coltsfoot and dandelion: Important medicinal plants from Asteraceae family. Aust. J. Basic Appl. Sci. 2011 ;12:1787–1791.

Mahmood I, Imadi SR, Shazadi K, Gul A, Hakeem KR. Effects of pesticides on environment, in: Plant, Soil and Microbes. Springer, Cham. 2016;253–269.

Hedges SA, Lacey MS. Pest Control Technology Field Guide for the Management of Structure-infesting Beetles. Franzak and Foster Co., Cleveland, OH. 1996;95.

Karaca K, Bilginturan M, Olgunsoy P. Effects of some plant essential oils against fungi on wheat seeds. Indian J. Pharm. Educ. Res. 2017;3:385–388.

Mishra RK, Bohra A, Kamaal N, Kumar K, Gandhi K, Sujayanand GK, Mishra M. Utilization of biopesticides as sustainable solutions for management of pests in legume crops: Achievements and prospects. Egypt. J. Biol. Pest Control. 2018;28(1):3.

Nefzi A, Abdallah BAR. Jabnoun-Khiareddine R, Saidiana-Medimagh S. Haouala R, Danmi-Remad M. Antifungal activity of aqueous and organic extracts from Withania somnifera L. against Fusarium oxysporum f.sp. radicis-lycopersici, J. Microbial Biochem. Technol. 2016;3:144–150.

Hikal WM, Baeshen RS, Said-Al Ahl HA. Botanical insecticide as simple extractives for pest control. Cogent Biol. 2017;3(1): 1404274.

Colovic MB. Krstic, Danijela Z., Lazarevic-Pasti, Tamara D, Bondzic, Aleksandra M. Vasic, Vesna M. Acetylcholinesterase inhibitors: Pharmacology and Toxicology. Current Neuropharmacology. 2013;11(3): 315–335.

Mizubuti ES, Júnior VL, Forbes GA. Management of late blight with alternative products. Pest Technol. 2007;2:106–116.

Srijita D. Biopesticides: An eco-friendly approach for pest control. World J. Pharm. and Pharm. Sci. 2015;6:250–265.

Gurjar MS, Shahid A, Akhtar M, Kangabam SS. Efficacy of plant extracts in plant disease management. Agric. Sci. 2012;3: 425–433.

Dubey NK, Shukla R, Kumar A, Singh P, Prakash B. Prospects of botanical pesticides in sustainable agriculture. Curr. Sci. 2010;98(4):479–480.

Dubey NK, Srivastava B, Kumar A. Current status of plant products as botanical pesticides in storage pest management. J. Biopestic. 2008;1(2):182–186.

Kole RK, Banerjee H, Bhattacharyya A. Monitoring of pesticide residues in farm gate vegetable samples in West Bengal. Pest Research Journal. 2002;14(1):77–82.

Hassanien MMM, Abdel-Razek AG, Rudzinska M, Siger A, Ratusz K, Przybylski R. Phytochemical contents and oxidative stability of oils from non-traditional sources. European Journal of Lipid Science and Technology. 2014; 116(11):1563-1571.

Dales MJ. A review of plant materials used for controlling insect pests of stored products. Natural Resources Institute (NRI) Bulletin 65, Overseas Development Administration. 1996;84.

Babayan VK, Kootungal D, Halaby GA. Proximate analysis Fatty acid and amino acid composition of Nigella sativa seeds. J Food Sci. 1978;43:1314-1315.

Sastry EV, Anan DM. Cumin, Fennel and Fenugreek. Soil, Plant Growth and Crop Production. Encyclopedia of Life Support Systems; 2013.

Bharat BA. Molecular Targets and Therapeutic Uses of Spices. Google Books; 2009;259.

ISBN 978-981-14468-95-4.

Karapinar M, Aktug SE. Inhibition of food borne pathogens by thymol, eugenol, menthol and anethole. Int J Food Microbiol. 1987;4 (2):161-166.

Archer MS, Elgar MA. Cannibalism and delayed pupation in hide beetles, Dermestes maculatus DeGeer (Coleoptera: Dermestidae). Australian Journal of Entomology. 1998;37:158-161.

Kulshrestha P, Satpathy DK. Use of beetles in forensic entomology. Forensic Science International. 2001;120:15-17.

Veer V, Negi BK, Rao KM. Dermestid beetles and some other insect pests associated with stored silkworm cocoons in India, including a world list of dermestid species found attacking this commodity. Journal of Stored Products Research. 1996;32:69-89.

Peacock ER. Dermestes peruvianus Cast, D. heamorrhoidalis Kust and other Dermestes species. (Coleoptera: Dermestedae). Entomol. 1975;111:1-14.

Richardson MS, Goff ML. Effects of temperature and intraspecific interaction on the development of Dermestes maculatus (Coleoptera: Dermestidae). Journal of Medical Entomology. 2001;38: 347-351.

Jones TM, McNamara KB, Colvin PGR, Featherston R, Elgar MA. Mating frequency, fecundity and fertilization success in the hide beetle, Dermestes maculatus. Journal of Insect Behavior. 2006;19:357-371.

Harborne JB. Phytochemical Methods: A guide to modern techniques of plant analysis. Chapman and Hall Ltd, London. 1973;49-188.

Harborne JB, William CA. Advances in flavonoid research since 1992. Phyto-chemistry. 2000;55:481-504.

Obadoni BO, Ochuko PO. Phytochemical studies and comparative efficacy of the crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global J. Pure Applied sci. 2002;8:203-208.

Elgayyar M, Draughon FA, Golden DA, Mount JR. Antimicrobial activity of essential oils from plant against selected pathogenic and saprophytic microorganisms. Journal of Food Protection. 2001;64:1019-1024.

Adedire CO, Ajayi TS. Assessment of the insecticidal properties of some plant extracts as grain protectants against maize weevil. Nigerian J. Entomol. 1996;13:93-101.

Don-Pedro KN. Fumigant toxicity is the major route of insecticidal toxicity of citrus peel essential oils. Pesticide Sci. 1996; 46(1):71-78.

Fatope MO, Nuhu AM, Mann A, Takeda Y. Cowpea weevil bioassay: A simple prescreen for plants with grain protectant effects. Int. J. Pest Mgt. 1995;41(2):84-86.

Gil A, De LaFuente EB, Lenardis AE, Lopez PM, Suarez SA, Bandoni A. Coriander Essential oil composition from two genotype grown in different conditions. Journal of Agriculture and Food chemistry. 2002;50:2870-2877.

Golob P, Webley DJ. The use of plants and minerals as traditional protectants of stored products. Natural Resource Institutes. 1980;138.

Okonkwo EU, Ewete FK. Comparative evaluation of Dennettia tripetala seed powder, Pirimiphos-methyl and Aluminum phosphide tablet against Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae) and their biochemical and organoleptic effects on stored maize. Food Sci. Technol. 1998;163-165.

Okonkwo EU, Ewete FK. Comparative evaluation of Dennettia tripetala seed powder, Pirimiphos-methyl and Aluminium phosphide tablet against Callosobruchus maculatus Fabricius (Coleoptera: Bruchidae) and their biochemical and organoleptic effects on stored cowpea. Journal of Agricul. Res. 2000;1(1):48-53.

Okonkwo EU, Okoye WI. The efficacy of four seed powders and the essential oils as protectants of cowpea and maize grains against infestation by Callosobruchus maculatus (Fabricius) (Coleoptera: Bruchidae) and Sitophilus zeamais (Motschulsky) (Coleoptera: Curculionidae) in Nigeria. Int. J. Pest Mgt. 42(3):143-146.

Grdiša M, Grši ´c, K. Botanical insecticides in plant protection. Agric. Conspect. Sci. 2013;2:85–93.

Mbaye NN, Sarr M, Guiye-Ndiaye A, Samb A, Sembene M. Repulsive and Biocide activities of leaves powder of Crataeva religiosa (Forst) on Dermestes spp. Associated with the Salty smoked dried fish. International Journal of Biosciences. 2014;4(1): 306-312.

Kahsai AW. Isolation and Characterization of Active Ingredients from Nigella sativa for Antibacterial Screening; Master's Thesis, Department of Chemistry, East Tennessee State University. 2002;67.

Shanmugam MK, Warrier S, Kumar AP, Sethi G, Arfuso F. Potential Role of Natural Compounds as Anti-Angiogenic Agents in Cancer. Curr. Vasc. Pharmacol. 2017;6: 503-519.

Materska M, Perucka I. Antioxidant activity of the main phenolic compounds isolated from hot chilli pepper fruit (Capisicum annum L.). Journal of Agriculture and Food Chemistry. 53(5):1750-1756.

Okonkwo EU, Okoye WI. Insecticidal activity of Dennetia tripetala Baker f. and Piper guineense Schum. and Thonn. Against Dermestes maculatus DeGeer (Coleoptera: Dermestidae) and Necrobia rufipes DeGeer (Coleoptera: cleridae) on dried fish. Nigerian Journal of Entomology. 2001;18:109-117.

Don-Pedro KN. Toxicity of some citrus peels to Dermestes maculatus Degeer and Callosobruchus maculatus (f.). J. Stored Prod. Res. 1985;21(1):31-34.

Jatau A, Majeed Q, Bandiya HM. Effects of five local Spices on the mortality and Development of Larvae of Dermestes maculates De Geer (Coleoptera: Dermestidae) reared on dried smoked fish. International Scholarly and Scientific Research and Innovation. 2014;1(2):145-150.

Adesina JM, Jose AR, Rajashekar Y. Bioefficacy of Clerendrum capitatum Willd) Schumachet, Thorn. (Lamiales: Verbenaceae) against Dermestes maculatus Degeer, 1774 (Coleoptera: Dermestidae) Larvae infestation on smoked catfish Clarias gariepinus (Burchell, 1822) (Siluriformes: Claridae). Brazilian Journal of Biological Sciences. 2016;3(5): 37-44.

Rahman MA, Teleb MA, Biswas MM. Evaluation of Botanical product as Grain protectant against Grain weevil, Sitophilus granarious (L) on Wheat. Asian Journal of Plant Sciences. 2003;2:501-504.

Rattan RS. Mechanism of action of insecticidal secondary metabolites of plant origin. Crop Prot. 2010;29(9):913–920.

Paul LS. Opium and its alkaloid. American Journal of Pharmaceutical of Education; 2002.

Archived from the original 2007.

Retrieved: December 2007.

Snigdha MK, Upadhaya KBM, Akhilesh KS, Rama PT, Vinod KT. Carbohydrate- Based Therapeutics: A Frontier in Drug Discovery and Development. 2016;10. 1016/B978:307-361.

Ahmad W, Shilpa S, Sanjay K. Phytochemical Screening and antimicrobial study of Euphorbia hirta extracts. J. Med. Plant Stud. 2017;2:183–186.

Mallikarijuna N, Kranthi KR, Jadhav DR, Kranthi S, Chandra S. Influence of foliar chemical compounds on the development of Spodoptera litura in interspecific derivatives of groundnut. J. Appl. Entomol. 2004;128:321-328.