ACC Deaminase Containing Plant Growth Promoting Agrobacterium larrymoorie Strain MZ 3-ABF Confers Tolerance to Drought Stress in Chickpea (Cicer arietinum L.) Seedlings
Asian Journal of Advances in Agricultural Research,
Plant growth-promoting rhizobacteria (PGPR) that produce 1-aminocyclopropane-1-carboxylate (ACC) deaminase can alleviate plant growth constraints caused by water scarcity. In the present study, six PGPR strains were evaluated to produce several plant growth promoting, and ACC deaminase enzyme isolated from the rhizosphere soil of Chickpea (Cicer arietinum) in arid regions of Telangana State, India. According to their 16S rDNA sequencing analysis, only one of the six strains, MZ 3-ABF, belongs to Agrobacterium larrymoorie. A drought tolerance experiment revealed two PGPR strains with high phosphate solubilization, nitrogen fixation, indoleacetic-3-acid (IAA), and ACC deaminase enzyme secretion potential were constrained to only MZ 3-ABF and MZ 5-ABF. One strain MZ 3-ABF was chosen for use in a pot experiment to assess their growth-promoting effects on chickpea under drought conditions. This PGPR strain inoculation into chickpea seedlings was expected to alleviate the overall growth inhibition caused by drought stress. The inoculation was thought to have the greatest growth-promoting effects. Inoculation with strain MZ 3-ABF altered plant height, root length, dry biomass, and net photosynthetic rate of leaves, allowing chickpea seedlings to cope with drought better. They had an indirect effect on the biochemical and physiological properties of chickpea seedlings in order to alleviate drought stress. These findings suggest that the MZ 3-ABF PGPR may be useful for effectively weakening the growth inhibition caused by drought in chickpea. The strain could also be used as effective bioinoculant to maintain pea quality.
- Plant growth promotion
- ACC deaminase
- drought stress
How to Cite
Stainforth DA, Aina T, Christensen C, Collins M, Faull N, Frame DJ, Kettleborough JA, Knight Martin SA, Murphy JM, Piani C, Sexton D, Smith LA, Spicer RA, Thorpe AJ, Allen MR. Uncertainty in predictions of the climate response to rising levels of greenhouse gases. Nature. 2005;433:403-406.
Vurukonda SSKP, Vardharajula S, Shrivastava M, Ali SkZ. Enhancement of drought stress tolerance in crops by plant growth promoting rhizobacteria. Microbiological Research. 2016;184:13-24.
Venkateswarlu B, Shanker AK. Climate change and agriculture: adaptation and mitigation strategies. Indian J Agron. 2009;54:226–230.
Teuling AJ, van Loon A, Seneviratne SI, Lehner I, Aubinet M, Heinesch B, Bernhofer C, Grünwald T, Prasse H, Spank U. Evapotranspiration amplifies European summer drought. Geophysical Research Letters. 2013;40(10):2071-2075.
Yang H, Huntingford C, Wiltshire A, Sitch S, Mercado L. Compensatory climate effects link trends in global runoff to rising atmospheric CO2 concentration. Environ. Res. Lett. 2019;14:124075.
Cohen I, Zandalinas SI, Huck C, Fritschi FB, Mittler R. Meta-analysis of drought and heat stress combination impact on crop yield and yield components. Physiol. Plant. 2021;171:66–76.
Adnan M, Fahad S, Zamin M, Shah S, Mian IA, Danish S, Zafar-ul-Hye M, Battaglia ML, Naz RMM, Saeed B, Saud S. Coupling Phosphate-Solubilizing Bacteria with Phosphorus Supplements Improve Maize Phosphorus Acquisition and Growth under Lime Induced Salinity Stress. Plants. 2020;9:900.
Tyagi P, Ranjan R. Comparative study of the pharmacological, phytochemical and biotechnological aspects of Tribulus terrestris Linn. and Pedalium murex Linn: An overview. Acta Ecologica Sinica; 2021.
Ali SkZ, Sandhya V, Minakshi G, Venkateswar Rao L, Venkateswarlu B. Effect of inoculation with a thermotolerant plant growth promoting Pseudomonas putida strain AKMP7 on growth of wheat (Triticum spp.) under heat stress. J of Plant Interactions. 2011;6(4):239 246.
Derkowska E, Paszt LS, Harbuzov A, Sumorok B. Root Growth, Mycorrhizal Frequency and Soil Microorganisms in Strawberry as Affected by Biopreparations. Advances in Microbiology. 2015;5:65-73. Available:http://dx.doi.org/10.4236/aim.2015.51007.
Siddikee MA, Zereen MI, Wu M, Zhang W, Dai CC. Phomopsis liquidambaris reduces ethylene biosynthesis in rice under salt stress via inhibiting the activity of 1-aminocyclopropane-1-carboxylate deaminase. Arch Microbiol. 2021;203(10): 6215-6229.
Ait Barka E, Nowak J, Clement C. Enhancement of chilling resistance of inoculated grapevine plantlets with a plant growth promoting rhizobacterium; Burkholderia phytofirmans strain PsJn. Appl Environ Microbiol. 2006;72:7246–7252.
Han HS, Lee KD. Plant growth promoting rhizobacteria effect on antioxidant status, photosynthesis, mineral uptake and growth of lettuce under soil salinity. Res J Agric Biol Sci. 2005;1:210–215.
Dell’ Amico E, Cavalca L, Andreoni V. Improvement of Brassica napus growth under cadmium stress by cadmium resistance rhizobacteria. Soil Biol Biochem. 2008;40:74–84.
Ali SkZ, Sandhya V, Grover M, Kishore N, Rao LV, Venkateswarlu B. Pseudomonas sp. strain AKM-P6 enhances tolerance of sorghum seedlings to elevated temperatures. Biol Fertil Soils. 2009; 46:45–55.
Glick BR, Jacobson CB, Schwarze MMK, Pasternak JJ. 1-aminocyclopropane-1- carboxylic acid deaminase plays a role in plant growth promotion by Pseudomonas putida GR12-2? In: Ryder, M.H., Stephens, P.M., and G.D Bowen, (eds). Improving plant productivity with rhizosphere bacteria. Adelaide: CSIRO. 1994a;150-152.
Glick BR, Jacobson CB, Schwarze MMK, Pasternak JJ. 1- aminocyclopropane-1- carboxylic acid deaminase mutants of the plant growth promoting rhizobacteria Pseudomonas putida GR12-2 do not stimulate canola root elongation. Can J Microbiol. 1994b;40:911-915.
Shaharoona B, Riffat B, Muhammad A, Zahir Ahmed Z, Zia-Ul, H. 1-Aminocylopropane-1-Carboxylate (Acc)- Deaminase Rhizobacteria Extenuates Acc-Induced Classical Triple Response In Etiolated Pea Seedlings. Pak J Bot. 2006;38(5):1491-1499.
Murugesan S, Manoharan C, Vijayakumar R, Panneerselvam A. Isolation and characterization of Agrobacterium rhizogenes from the root nodules of some leguminous plants. International Journal of Microbiological Research. 2010;1(3):92-96.
Sandhya V, Ali SkZ, Grover M, Reddy G, Venkateswarlu B. Alleviation of drought stress effects in sunflower seedlings by the exopolysaccharides producing Pseudomonas putida strain GAP-P45. Biol Fertil Soils. 2009;46:17-26.
Fiske CH, Subbarow Y. A colorimetric determination of phosphorous. J Biol Chem. 1925;66:375–400.
Vurukonda Sai Shiva Krishna Prasad, Sandhya Vardharajula, Manjari Shrivastava, Ali SkZ. Multifunctional Pseudomonas putida strain FBKV2 from arid rhizosphere soil and its growth promotional effects on maize under drought stress. Rhizosphere. 2016;1(1):4-13.
Yaghoubi Khanghahi M, Strafella S, Allegretta I, Crecchio C. Isolation of Bacteria with Potential Plant-Promoting Traits and Optimization of Their Growth Conditions. Curr Microbiol. 2021;78(2): 464-478.
Tahiri AI, Raklami A, Bechtaoui N, Mohamed A, Abderrahim B, Khalid O, Abdelilah M. Beneficial Effects of Plant Growth Promoting Rhizobacteria, Arbuscular Mycorrhizal Fungi and Compost on Lettuce (Lactuca sativa) Growth under Field Conditions. Gesunde Pflanzen, 2022;74:219–235.
Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of Siderophore. Anal Biochem. 1987;160:47–56.
Kim HS, Kim JY, Lee SM, Park HJ, Lee SH, Jang JS, Lee MH. Isolation and characterization of indole-3-acetic acid- And 1-aminocylopropane-1-carboxylyic acid deaminase-producing bacteria related to environmental stress. Microbiology and Biotechnology Letters. 2019;47(3):390-400.
Kumawat KC, Poonam S, Sharon N, Gupta RK, Asmita S, Ramakrishnan MN, Bindumadhava H, Sudeep S. "Dual microbial inoculation, a game changer?–Bacterial biostimulants with multifunctional growth promoting traits to mitigate salinity stress in Spring Mungbean. Frontiers in microbiology. 2021;11:600576.
Penrose DM, Glick B.R. Methods for isolating and characterizing ACC deaminase-containing plant growth-promoting rhizobacteria. Physiol Plant. 2003;118:10–15.
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric methods for determination of sugars of related substances. Anal Chem. 1956;28:350–356.
Bates LS, Waldren RD, Teare ID. Rapid determination of free proline for water stress studies. Plant Soil. 1973;39:205–207.
Barnes JD, Balaguer L, Maurigue E, Elvira S, Davison AW. A reappraisal of the use of DMSO for the extraction and determination of chlorophyll “a” and “b” in lichens and higher plants. Environ Exp Bot. 1992; 32:87–99.
Ibrahim AM, Quick JS. Heritability of Heat Tolerance in winter and Spring Wheat. Crop Science. 2001;41:1401-1405.
Rezaei-Chiyaneh E, Mahdavikia H, Subramanian S, Alipour H, Siddique KH, Smith DL. Co-inoculation of phosphate-solubilizing bacteria and mycorrhizal fungi: Effect on seed yield, physiological variables, and fixed oil and essential oil productivity of ajowan (Carum copticum L.) under water deficit. Journal of Soil Science and Plant Nutrition, 2021;21(4):3159-3179.
Salam A, Khan AR, Liu L, Yang S, Azhar W, Ulhassan Z, Gan Y. Seed priming with zinc oxide nanoparticles downplayed ultrastructural damage and improved photosynthetic apparatus in maize under cobalt stress. Journal of Hazardous Materials, 2022;423:127021.
Nayyar H, Gupta D. Differential sensitivity of C3 and C4 plants to water deficit stress: association with oxidative stress and antioxidants. Environmental and Experimental Botany. 2006;58(1-3):106-113.
Chen WP, Kuo TT. A simple and rapid method for the preparation of Gram- negative bacteria genomic DNA. Nucleic Acids Res. 1993;21:2260.
Lucy M, Reed E, Bernard RG. Applications of free living plant growth-promoting rhizobacteria. Antonie van leeuwenhoek. 2004;86(1):1-25.
Heidstra R, Yang WC, Yalcin Y, Peck S, Emons AM, Kammen van A, Bisseling T. Ethylene provides positional information on cortical cell division but is not involved in Nod factor-induced root hair tip growth in Rhizobium-legume interaction. Development. 1997;124(9):1781–1787.
Glick BR, Penrose DM, Li J. A model for the lowering of plant ethylene concentrations by plant growth promoting bacteria. J Theor Biol. 1998;190:63–68.
Mayak S, Tirosh T, Glick BR. Plant growth-promoting bacteria that confer resistance to water stress in tomato and pepper. Plant Sci. 2004a;166:525–530.
Mayak S, Tirosh T, Glick BR. Plant growth-promoting bacteria confer resistance in tomato plants to salt stress. Plant Physiol Biochem. 2004b;42:565–572.
Grichko VP, Glick BR. Amelioration of flooding stress by ACC deaminase-containing plant growth-promoting bacteria. Plant Physiol Biochem. 2001; 39:11–17.
Burd GI, Dixon DG, Glick BR. A plant growth-promoting bacterium that decreases nickel toxicity in seedlings. Appl Environ Microbiol. 1998;64:3663–3668.
Wang C, Knill E, Glick BR, Défago G. Effect of transferring 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase genes into Pseudomonas fluorescens strain CHA0 and its gacA derivative CHA96 on their growth-promoting and disease-suppressive capacities. Can J Microbiol. 2000;46:898–907.
Campbell BG, Thomson JA. 1-Aminocyclopropane-1-carboxylate deaminase genes from Pseudomonas strains. FEMS Microbio Lett. 1996;138: 207–210.
Hontzeas N, Zoidakis J, Glick BR, Abu-Omar MM. Expression and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the rhizobacterium Pseudomonas putida UW4: A key enzyme in bacterial plant growth promotion. Biochim Biophys Acta, 2004a; 1703:11–19.
Shah S, Jipingm L, Barbaram AM, Bernardm RG. Isolation and characterization of ACC deaminase genes from two different plant growth-promoting rhizobacteria. Can J Microbiology. 1998;44 (9):833-843. Available:https://doi.org/10.1139/w98-074.
Babalola OO, Osir EO, Sanni AI, Odhiambo GD, Bulimo WD. Amplification of 1-amino-cyclopropane-1-carboxylic (ACC) deaminase from plant growth promoting bacteria in Striga-infested soil. Afr J Biotechnol. 2003;2:157–160.
Jacobson CB, Pasternak JJ, Glick BR. Partial purification and characterization of 1-aminocyclopropane-1-carboxylate deaminase from the plant growth promoting rhizobacterium Pseudomonas putida GR12-2. Can J Microbiol. 1994; 40:1019–1025.
Sheehy RE, Honma M, Yamada M, Sasaki T, Martineau B, Hiatt WR. Isolation, sequence, and expression in Escherichia coli of the Pseudomonas sp. strain ACP gene encoding 1-aminocyclopropane-1-carboxylate deaminase. J Bacteriol. 1991; 173:5260-5265.
Sandhya V, Ali SkZ, Grover M, Reddy G, Venkateswarlu B. Effect of plant growth promoting Pseudomonas spp. on compatible solutes, antioxidant status and plant growth of maize under drought stress. Plant Growth Regul. 2010;62:21-30.
Minakshi G, Madhubala R, SkZ Ali, Yadav SK, Venkateswarlu B. Influence of Bacillus spp. strains on seedling growth and physiological parameters of sorghum under moisture stress conditions. J Basic Microbiol. 2013;53:1–11.
Seki M, Narusaka M, Abe H, Kasuga M, Yamaguchi-Shinozaki K, Carninci P, Hayashizaki Y, Shinozaki K. Monitoring the expression pattern of 1300 Arabidopsis genes under drought and cold stresses by using a full-length cDNA microarray. Plant Cell. 2001;13:61–72.
Arshad M, Frankenberger WT Jr. Ethylene: agricultural sources and applications. Annals of Botany. 2002;90(3):424.
Glick BR, Patten CL, Holguin G, Penrose GM. Biochemical and Genetic Mechanisms Used by Plant Growth Promoting Bacteria Imperial College Press, London, 1999.
Marcelis L, Van Hooijdonk J. Effect of salinity on growth, water use and nutrient use in radish (Raphanus sativus L.). Plant and Soil. 1999;215:57–64.
Delauney A, Verma S. Proline biosynthesis and osmoregulation in plants. Plant J. 1993;4:215–223.
Nanjo T, Kobayashi M, Yoshiba Y, Sanada Y, Wada K, Tsukaya H, Kakubari Y, Yamaguchi-Shinozaki K, Shinozaki K. Biological functions of proline in morphogenesis and osmotolerance revealed in antisense transgenic Arabidopsis thaliana. Plant J. 1999;18: 185–193.
Sung Dong-Yul, Fatma K, Kil-Jae L, Charles LG. Acquired tolerance to temperature extremes. Trends in Plant Sciences. 2003;8(4):179-187.
Ashraf M, Shahzad SM, Imtiaz M, Rizwan MS. Salinity effects on nitrogen metabolism in plants-focusing on the activities of nitrogen metabolizing enzymes: A review. J. Plant Nutr. 2018; 41:1065–1081.
Helton JC, Johnson JD, Sallaberry CJ, Storlie CB. Survey of sampling-based methods for uncertainty and sensitivity analysis. Reliability Engineering & System Safety. 2006;91(10-11):1175-1209.
Sairam RK, Tyagi A. Physiology and Molecular Biology of Salinity Stress Tolerance in Plants. Current Science. 2004;86:407-421.
Hall JA, Peirson D, Ghosh S, Glick BR. Root elongation in various agronomic crops by the plant growth promoting rhizobacterium Pseudomonas putida GR12-2. Isr J Plant Sci. 1996;44:37–42.
Abstract View: 83 times
PDF Download: 26 times