In vitro Plant Regeneration in Banana (Musa sp.) cv. Grand Nain
Aiman Yousaf
Department of Chemistry, Islamia University of Bahawalpur, Pakistan.
Muhammad Ijaz *
Institute of Biochemistry, Biotechnology and Bioinformatics, Islamia University of Bahawalpur, Pakistan.
Hamza Iftikhar
Institute of Biochemistry, Biotechnology and Bioinformatics, Islamia University of Bahawalpur, Pakistan.
Aqsa Mustafa
Institute of Biochemistry, Biotechnology and Bioinformatics, Islamia University of Bahawalpur, Pakistan.
Nerum Tasneem
Institute of Biochemistry, Biotechnology and Bioinformatics, Islamia University of Bahawalpur, Pakistan.
Muhammad Sultan
Biological Science Department, COMSATS University Islamabad, Pakistan.
Imran Javed *
Department of Biotechnology, COMSATS University, Abbottabad Campus, Islamabad, Pakistan.
Zeeshan Aslam
Agriculture Department, The Islamia University of Bahawalpur, Pakistan.
Maria Fatima
Department of Zoology, The Islamia University of Bahawalpur, Pakistan.
*Author to whom correspondence should be addressed.
Abstract
This study was conducted at the tissue culture laboratory in Pakistan. Suckers obtained from the banana cv. Grand Nain to explore the effects of different concentrations of BAP and IAA. After inoculation with Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) and the Indole-3- acetic acid (IAA) the suckers first turn into the brown after 5-10 days then turned into a green mass-like structure after 30 days. From this mass-like structure shoots and roots were developed. Among the different concentrations, BAP 7.5 mg/l and IAA 0.5 mg/l showed the highest number of shoots 0.75, 2.75 and 6.25 per explant and shows the highest shoot lengths which 1.03 cm, 2.45 cm and 3.40 cm respectively. IAA is essential for only roots proliferation. The roots of different lengths were produced when the concentration of IAA 0.5 mg/l was used and 2.93 cm, 4.63 cm and 5.88 cm roots length produced at 15, 30 and 45 days after occultation respectively. Then these plants were transferred to the greenhouse for hardening acclimatization with the environment, the hardening process took place, and the established plantlets are ready for planting.
Keywords: Suckers, shoots, roots, hardening, plantlets