In vitro Regeneration of Potato (Solanum tuberosum L.) Using Sprout Explant
Md. Sabbir Hossain
Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
M. Hasanuzzaman
Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
Md. Arifuzzaman *
Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
Md. Hassanur Rahman
Department of Horticulture, Hajee Mohammad Danesh Science and Technology University, Dinajpur-5200, Bangladesh.
*Author to whom correspondence should be addressed.
Abstract
In vitro regeneration ability of four varieties of potato (Solanum tuberosum) was evaluated for plantlet production applying four hormonal concentrations and combinations. Shoot and root characteristics were observed at 15 days after inoculation of sprout explants. There was significant variation found among the four treatments in four studied traits. The genotype Chollisha exhibited superior performance in plantlet production among four genotypes. Out of four hormonal teatments, T3 {MS+ 2, 4- D (2 mg/l) + BAP (2 mg/l)} hormonal combination showed high effectiveness for in vitro plantlet regeneration. Therefore, this protocol might be used in future micropropagation of potato for large scale potato plantlet production.
Keywords: In vitro, plantlet, regeneration, potato