Enhancement of Growth Potential of Duranta repens L. by Ethanolic Extract Fractions of the Cleome gynandra L.
Yanelis Avilés-Tamayo
Department of Biological Sciences, University of Granma, Cuba
Yoannia Pupo-Blanco
Department of Biological Sciences, University of Granma, Cuba
Yosvel Viera-Tamayo *
Studies Center of Plant Biotechnology (CEBVEG), University of Granma, Cuba
Úrsula Rosabal-Cordoví
Quality Laboratory, Company of Oral Liquid Drugs (MEDILIP), Granma, Cuba
Yans Guardia-Puebla
Department of Mechanical Engineering, University of Granma, Cuba
Carlos Sangoquiza-Caiza
National Institute of Agricultural Sciences (INIAP), Quito, Ecuador
Edwin Naranjo-Quinaluisa
National Institute of Agricultural Sciences (INIAP), Quito, Ecuador
*Author to whom correspondence should be addressed.
Abstract
The study was conducted with the objective of investigating the ethanolic extract fractions of the stem of Cleome gynandra L. by analyzing its bioactivity in stakes of Duranta repens, L. The extracts were separated from a dry ethanolic extract (20% tincture m/V) with the solvents ethyl ether, chloroform, ethyl acetate and distilled water and evaluated at doses of 50, 100, 150 mg m L-1, compared to a control to which only sterile distilled water was applied. The variables measured in D. repens at 45 days of cultivation were: number of shoots, length of shoots, and number of leaves in the apical buds, number of roots and length of the first root formed. In the different fractions evaluated, a stimulating effect was presented in the selected variables; therefore, the presence of bioactive metabolites of different polarity significatively influences the growth potential of C. gynandra. However, better results of plant growth were obtained in solvents of higher polarity, especially the acetate and aqueous fractions.
Keywords: Stimulating activity, ethanolic extract, Cleome gynandra L, Duranta repens L