Germination Indices and Seedling Growth Parameters in Maize under Salinity Stress with Varying Concentrations of Giberellic Acid (GA)

Hanim Mohi EldenAhmed Matter

Department of Biology, Faculty of Education Gadarif University, Sudan.

Badr ELdin Abdelgadir Mohamad Ahmed *

Department Crop Sciences, Faculty of Agriculture, Kassala University, Sudan.

Abdelmalik Omar Ahmed Idris

Department of Biology, Faculty of Education Gadarif University, Sudan.

*Author to whom correspondence should be addressed.


Salinity stress is one of the factors limiting seed emergence and seedling development in sunflower at New halfa scheme in East of Sudan. A study of two factors pots experiment was conducted during winter season of 2019 to evaluate germination indices and seedling growth characters of maize seeds under salinity stress with varying concentrations of synthetic growth regulator namely (GA3). The experiment was laid out according RCBD with three replications. Treatments consisted of five levels of salinity (0, 0.5, 1.0, 1.5 and 2% ) designated as Na0, Na0.5, Na1.0 ,Na1.5 and Na2.0, and  four levels of GA3 were (0, 50,75 and 100 designated as G0, G50, G75 and G100, respectively,. The studied parameters include germination indices; daily germination (MDG), daily germination speed (DGS), final germination percentage (FGP), mean emergence time (MET), mean Energy of germination (EE), emergence index (EI), coefficient of uniformity of emergence (CUE) and vigour index (SVI). Also, seedling fresh and dry weight, shoot length and root length were measured, and the shoot/root ratio was calculated. The negative effects of salinity stress significantly decreased germination indices and seedlings growth characters in a dose dependent manner. The results showed that, application of GA3 at medium levels concentration (specify those doses) increased all studied parameters. In conclusion, application of high Ga3 levels enhanced all germination indices and seedlings growth characters while high salinity levels reduced these traits of maize seeds. Such result has made the base for further study that should be conducted in different seasons with different growth regulators involving different parameters of other crops.  

Keywords: Maize, Gabralline, salinity, germination indices, seedling vigor

How to Cite

Matter, Hanim Mohi EldenAhmed, Badr ELdin Abdelgadir Mohamad Ahmed, and Abdelmalik Omar Ahmed Idris. 2024. “Germination Indices and Seedling Growth Parameters in Maize under Salinity Stress With Varying Concentrations of Giberellic Acid (GA)”. Asian Journal of Advances in Agricultural Research 24 (6):82-88.


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Guelloubi R., Hamdy A, Sardo V. Maize production under two water saving techniques. El Gamal F. (ed.), Lamaddalen a N.(ed.), Bogliotti C.(ed.), Guelloubi R.(ed.). Nonconventional water use: WASAMED project. Bari; 2005.

Xue X, Du S, Jiao F, Xi M, Wang A, Xu H, Jiao Q, Zhang X, Jiang H, Chen J. The regulatory network behind maize seed germination: Effects of temperature, water, photo hormones, and nutrients. Crop J. 2021;9:901–914

Yang B, Yin Y, Liu C, Zhao Z, Guo M. Effect of germination time on the compositional, functional and antioxidant properties of whole wheat malt and its end-use evaluation in cookie-making. Food Chem. 2021;349:129125.

Aubertot JN, Deguine JP, Lamichhane JR, Robin MH, Sarthou JP, Steinberg C. Vers Une Protection Agroécologique Des Cultures En Phase d’implantation. In Réussir l’Implantation Des Cultures. Enjeux Agroécologiques, Itinéraires Techniques; Quae: Paris, France. 2022; 107–134.

Liu Y, Ye N, Liu R, Chen M, Zhang J. H2O2 mediates the regulation of ABA catabolism and GA biosynthesis in Arabidopsis seed dormancy andgermination. Journal of Experimental Botany. 2010;61:2979-2990

Ali E, Hussain N, Shamsi IH, Jabeen Z, Siddiqui MH, Jiang L. Role of jasmonic acid in improving tolerance of rapeseed (Brassica napus L.) to Cd toxicity. Journal of Zhejiang University: Science B (Biomedicine & Biotechnology). 2018;19 (2):130-146.

CIHEAM/EU DG Research, 77-91.

Zhang C, Luo W, Li Y, Zhang X, Bai X, Niu Z. Transcriptomic analysis of seed germination under salt stress in two desert sister species (Populus euphratica and P. pruinosa). Frontiers in Genetics. 2019; 10:1-16.

Bawa G, Feng L, Chen G, Chen H, Hu Y, Pu T, Wang X. Gibberellins and auxin regulate soybean hypocotyls elongation under low light and high temperature interaction. Physiol. Plant. 2020;170(3): 345-356. Available: ppl.1315

Pangapanga-Phiri I, Mungatana ED. Adoption of climate-smart agricultural practices and their influence on the technical efficiency of maize production under extreme weather events. Int. J. Disaster Risk Reduct. 2021;61: 102322.

Ali AS, Elozeiri AA. Metabolic processes during seed germination. in advances in seed biology; InTech: Vienna, Austria.3. Gummerson, R.J. The Effect of Constant Temperatures and Osmotic Potentials on the Germination of Sugar Beet. J. Exp. Bot. 2017;37:729–741.

Hussain S, Khaliq A, Matloob A, Wahid M. Afzal I. Germination and growth response of three wheat cultivars to NaCl salinity. Soil Environ. 2013;32(1):36-43

Salehifar M, Torang A, Farzanfar M, Salehifar M. Comparison of Salinity stress effect on germination and seedling growth in 8 lines genotypes of common bean (Phaseolus vulgaris). 11th Iranian Crop Science Congress. 2010;3794.

Jenks MA (eds.), Advances in Molecular Breeding Toward Drought and Salt Tolerant Crops, 2007:1–32

Rodríguez Coca LI, García González MT, Gil Unday Z, Jiménez Hernández J, Rodríguez Jáuregui MM, Fernández Cancio Y. Effects of Sodium Salinity on Rice (Oryza sativa L.) Cultivation: A Review. Sustainability. 2023;15:1804. Available:

Dai F, Rong Z, Wu Q, Abduallah EF, Liu C, Shengrui L. Mycorrhiza improves plant growth and photosynthetic characteristics of tea plants in response to drought stress. BIOCELL. 2022;46: 1339–1346. DOI: 10.32604/biocell. 018909

Elias SM, Hossain MS, Sikder FS, Ahmed J, Karim MR. Identification of constraints to pulseproduction with special reference topresent farming systems. Annual Report of the Agricultural Economic Division, BARI, Joydebpur. 1986:1.

Farooq M, Basra SMA, Khalid M, Tabassum R, Mehmood T. Nutrietn homeostasis, reasrvers metabolism and seedling vigor as affected by seed priming in coarse rice. Candadian Journal of Botany. 2006;84:1196–1202.

Basra SMA, Iftikhar MN, Afzal I. Potential of moringa (Moringa oleifera) leaf extract as priming agent for hybrid maize seeds. International Journal of Agriculture and Biology. 2011;13:1006–1010.

Bewley JD, Black M. Seeds: Physiology of Development and Germination. Plenum Press, New York. 1985:445.

Panel ATM, Tinjmul Islam, Thawatchai Koeds, Hayat Ullah, Sujin Jenweerawat, Suriyan Cha-um. Salt tolerance of hybrid baby com genotype in related of growth yield, physiological, and biochemical characters. South African Journal of Botany. 2022;147:808-819.

Turkyilmaz B. Effects of salicylic and gibberellic acids on wheat (Triticum aestivum l.) under salinity stress. Bangladesh J. Bot. 2012;41(1): 29-34.

Wittwer SH, Bukovac MJ. The effects of gibberellins on economic crops. Econ.Bot. 1958;12: 213-255.

Rodríguez Coca LI, García González MT, Gil Unday Z, Jiménez Hernández J, Rodríguez Jáuregui MM, Fernández Cancio Y. Effects of Sodium Salinity on Rice Cultivation: A Review. Sustainability 2023;15:1804. Available: 10. 3390/su150 31804

Sheldon A, Menzies NW, So HB, Dalal R. The effect of salinity on plant available water. Paper presented at the SuperSoil 2004:3rd Australian New Zealand Soils Conference, University of Sydney, Australia. 2004:5-9.

Asad A, Blamey FPC and Edward SDG. Effects of boron foliar applications on vegetative and repro ductile growth of sunflower. Ann Bot. 2003;92: 565- 570.