Open Access Original Research Article
This study was conducted at the tissue culture laboratory in Pakistan. Suckers obtained from the banana cv. Grand Nain to explore the effects of different concentrations of BAP and IAA. After inoculation with Murashige and Skoog (MS) medium supplemented with different concentrations of 6-Benzylaminopurine (BAP) and the Indole-3- acetic acid (IAA) the suckers first turn into the brown after 5-10 days then turned into a green mass-like structure after 30 days. From this mass-like structure shoots and roots were developed. Among the different concentrations, BAP 7.5 mg/l and IAA 0.5 mg/l showed the highest number of shoots 0.75, 2.75 and 6.25 per explant and shows the highest shoot lengths which 1.03 cm, 2.45 cm and 3.40 cm respectively. IAA is essential for only roots proliferation. The roots of different lengths were produced when the concentration of IAA 0.5 mg/l was used and 2.93 cm, 4.63 cm and 5.88 cm roots length produced at 15, 30 and 45 days after occultation respectively. Then these plants were transferred to the greenhouse for hardening acclimatization with the environment, the hardening process took place, and the established plantlets are ready for planting.
Open Access Review Article
Nowadays most people in advanced countries endangered under adult diseases such as hypertension, diabetes, hyperlipidemia, inflammatory disease, human oxidation, and liver-related diseases. In South Korea also very serious situation for the adults and children by the changing from vegetable food to animal food and changed labor pattern from physical labor to mental labor.
We aim to review Protaetia brevitarsis surensis (PBS)’s beneficial multi-functions on human with research reports and verification of well-known common sense in South Korea, which was commonly known as medicinally very effective in liver related diseases. PBS larvae known as cold body characteristics, so, it more effective to Yang-in(Oriental physical constitution classification method: Warm body which Jinseng, Panax ginseng, not available people) than Um-in(Cool body- Jinseng available people). The functional effects mostly known effective on liver related diseases mainly by oriental clinic and as folk remedies. However, nowadays PBS larvae’s folk remedies and other multi-functions scientifically researched and that results verified by many papers such as whitening of skin therapeutic effects, prevention of breast cancer, inflammatory disease, human oxidation, and liver-related diseases. For the increasing of medicinal effects, advanced fermentation and ethanol extraction methods will be very useful compared to non-fermentation of PBS larvae and water extraction. So, we concluded that PBS could diminish or decreasing the adult diseases and it will be very useful food and medicinal materials simultaneously for the human food and medicine by the advanced extraction methods.